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ConclusionsThe developed LAT and flow through assays were simple, rapid and economical for the detection of leptospira infection and suitable for large scale screening of samples in endemic areas without any sophisticated equipment. KeywordsDiagnosis, flow through assay, latex agglutination test, leptospirosis, rLipL41Humoral immunity prevents clinical malaria during Author summary Plasmodium vivax contributes significantly to global malaria morbidity and remains a major obstacle for malaria elimination due to its ability to form dormant stages in the liver. These forms can become activated to cause relapsing blood stage infections. Relapses remain poorly understood because it is difficult to verify whether P. vivax blood stage infections in patients are